The Basic Principles Of high performance liquid chromatography
The Basic Principles Of high performance liquid chromatography
Blog Article
In the ionization chamber the remaining molecules—a mixture with the cell period elements and solutes—endure ionization and fragmentation. The mass spectrometer’s mass analyzer separates the ions by their mass-to-cost ratio (m/z). A detector counts the ions and shows the mass spectrum.
Rotating the internal valve (demonstrated in crimson) for the inject position directs the cell period through the sample loop and on to the column.
This web site doesn't exist as part of your selected language. Your preference was saved and you will be notified after a site is usually viewed inside your language.
Keep in mind, consulting your instrument manual and also the company's specialized help can also be valuable sources when troubleshooting specific difficulties with all your HPLC system.
物質にエネルギーを与える（励起）ことにより発光する（蛍光）性質を利用した検出器。一般に選択性が高く高感度で、物質に特異的な検出が可能。蛍光する性質を持たない物質については、その物質を標識することにより検出が可能になる。
A detector identifies and measures each element. Retention time suggests enough time taken for every compound to exit the column. HPLC's efficiency is determined by elements like column form and mobile stage composition. Frequent maintenance assures exact final results. Knowledge HPLC's step-by-move approach is significant for precise chemical Examination in laboratories.
ⅱ. 액체 크로마토그래피 정보에 대해 더 자세한 내용은 크로마토그래피 학습센터를 참고해주세요.
-hydroxybenzoic acid elutes a lot more slowly. Though we are able to solve entirely both of these solutes applying mobile section that's 16% v/v acetonitrile, we can't resolve them When the mobile section is ten% tetrahydrofuran.
four. If your peaks for fluoxetine and protriptyline are settled insufficiently, how could possibly you alter the cellular phase to enhance their separation?
移動相としては、カラムや装置に悪影響を与えない範囲で各種の溶媒が使用される。水や塩類の水溶液、アルコール類、アセトニトリル、ジクロロメタン、トリフルオロ酢酸などが用いられる。相溶性のある（互いに混じり合う）溶媒を混合して使用する場合が多い。
Conversely, a stream price that is far too reduced may cause excessive band broadening. Check your stream fee configurations and modify them according to the proven method.
Common cell section modifiers like acids and bases read more can be additional to fantastic-tune the conversation involving analytes and the column. These modifiers can:
Immediately after loading the sample, the injector is turned on the inject position, which redirects the mobile stage through the sample loop and onto the column.
, we are able to location a solvent proportioning valve just before one pump. The solvent proportioning benefit connects two or even more solvent reservoirs into the pump and establishes just how much of each and every solvent is pulled throughout Every single with the pump’s how HPLC works cycles. An additional strategy for getting rid of a pulsed circulation is to incorporate a pulse damper amongst the pump as well as the column.